Resources

Techniques Developed or Improved

by the Sive Lab

• Xenopus Lab Manual (Hazel Sive, Editor-in-Chief)
• Simplified subtractive cDNA cloning
• Hormone-inducible protein function in Xenopus
• Zebrafish embryo explant analysis
• Haploid screening in zebrafish
• Live confocal imaging of zebrafish brain
• RNAi in zebrafish
• Zebrafish brain ventricle injection
• Cerebrospinal fluid drainage in zebrafish
• Permeability assays for zebrafish neuroepithelium
• Protein knockout validation by in vitro translation
• Translational inhibition by polyC tracts
• Facial transplants in Xenopus
• Non-invasive multielectrode array analysis in zebrafish

Sive Lab Review Articles

Relevant to Key Fields

• Brain ventricular system
  • https://pubmed.ncbi.nlm.nih.gov/19274662/
  • https://pubmed.ncbi.nlm.nih.gov/32078177/
  • https://pubmed.ncbi.nlm.nih.gov/15327780/
• Mental health disorders in model organisms
  • https://pubmed.ncbi.nlm.nih.gov/14606692/
  • https://pubmed.ncbi.nlm.nih.gov/26092527/
  • https://pubmed.ncbi.nlm.nih.gov/26002061/
• Keeping two animal systems in one lab: a frog and fish case study
  • https://pubmed.ncbi.nlm.nih.gov/21805281/
•  Tool vs Model organisms
  • https://pubmed.ncbi.nlm.nih.gov/21357758/
•  Education
  • https://pubmed.ncbi.nlm.nih.gov/23868246/